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The second response cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to create ferric enzyme, NO, citrulline, and water special info as items (Fig. 9) - circularly polarized luminescence. As drawn in Fig - https://sketchfab.com/olisclarity1. 9, the reaction may also include electron transfer from H4B to the Fe, IIO2 intermediate

l9 As displayed in Fig. 9, this could be because the H4B extreme acts as an electron sink in a later point in the response, so as to allow NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.